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        Center for Molecular Medicine

        Dedicated to understanding the intricacies of cellular signaling, the Center for Molecular Medicine was built on Caltech's successes at the interface of chemistry and biology. It is focused on determining how complex systems of molecules interact to create the pathways that regulate the lives of cells and allow them to respond to their environments. Rather than just studying a complex biomolecule in isolation, an understanding of how it is part of a larger path of molecules is the purpose of its establishment. The Center was made possible by a generous grant from the Gordon and Betty Moore Foundation. 

        If use of the Center instrumentation has contributed significantly to your research, we request that you cite the Center in the acknowledgments section of your publication.  For example: "This work was supported by…, and by the Center for Molecular Medicine at California Institute of Technology."

        Instrumentation:

        Crellin Room 260 (Bacterial)

        Crellin Room 262 (Mammalian)

        How to get access to CM2 and use the instruments in CM2?

        1. Contact the appropriate caretakers for each instrument you would like to use for the instrument training.

        2. Upon completion of training, contact Dina Malounda (malounda@) to get access.


        People in the Facility:

        • Faculty members: 
          Dr. Dennis Dougherty
          Dr. Jacqueline Barton
          Dr. Peter Dervan
          Dr. Linda Hsieh-Wilson
          Dr. Shu-ou Shan
          Dr. Alison Ondrus
          Dr. Mikhail Shapiro
           
        • Lab Assistants:
          Please see the table of equipment caretakers

        • Safety Officer
          Dina Malounda malounda@
           

        Seminars:

        Thursday afternoons
        151 Crellin
        12:00 to 1:00 p.m.
        Upcoming Seminars:

        January 31, 2019 - David Romney
        February 7, 2019 - Rebekah Silva
        February 14, 2019 - Patrycja Kielb
        February 21, 2019 - Elliot Mackrell
        February 28, 2019 - George Mobbs
        March 7, 2019 - Arash Farhadi
        March 14, 2019 - Christopher J. Bley

        Information on Instrumentation:


        For training or troubleshooting, please contact the appropriate caretakers.


        Equipment

        Caretaker

        Contact info

        Biosafety cabinet (TC hood)

        Alice Hsu

        ahhsu@

        Chemical fume hood

        Dina Malounda

        malounda@

        Amaxa 4-D Nucleofector

        John Thompson

        jthompso@

        Benchtop centrifuges

        Dina Malounda

        malounda@

        Benchtop water bath

        Alice Hsu

        ahhsu@

        MilliQ water deionizer

        Brendan O'Boyle

        bmoboyle@

        Typhoon FLA 9000 gel imager

        Siobhan MacArdle

        smacardl@

        Inverted fluorescence microscope (Nikon TS100)

        Kun Miao

        kmiao@

        Upright dissection microscope (Nikon SMZ1000) + Clean bench

        John Thompson

        jthompso@

        Affinity ITC

        Stefan Petrovic

        spetrovi@

        Mammalian cell incubator

        Kun Miao

        kmiao@

        BioRad qPCR (CFX 96 RT PCR)

        Wen Zhou

        wzhou@

        4°C refrigerator

        Alice Hsu

        ahhsu@

        FlexStation microplate reader

        Stephen Grant

        sgrant@

        Beckman Avanti J-26 floor centrifuge

        Alex Siegel

        asiegel@

        -80°C freezer

        Brendan O'Boyle

        bmoboyle@

        Nanodrop 2000

        Dan Zhou

        dzhou@

        Li-Cor IR imager

        Chien-I Yang

        cyang3@

        Beckman Scintillation Counter

        Rebekah Silva

        rsilva@

        Shaking incubator for bacteria

        Terry Kim

        tdkim@

        Ultrospec cell density meter

        John Thompson

        jthompso@

        QIAcube

        Xun Wang 

        Xwang2@

        Neon transfection system

        Gracie Zhang

        gzhang@

        Ice maker

        Trixia Buscagan

        tbuscaga@

        Flexstation 3

        Microplate reader with 5 read modes (including absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence) with additional robot functionality (8-channel pipettor) to add liquid reagents.

        Contact: Stephen Grant, sgrant@

        cellsig_flexstation.jpg

        Odyssey Li-Cor IR Imager

        Quantitative scanning of western blots at 700 and 800 nm.

        Contact: Chien-I Yang, cyang3@

        cellsig_odyssey.jpg

        CFX 96 Real-Time PCR Detection System

        Built upon the Bio-Rad C1000 96 well Thermal Cycler, utilizes filtered LEDs for excitation and is capable of monitoring 450-750 nM excitation/emission wavelength range. This system allows for simultaneous detection of up to 5 different fluorophores per reaction and is sensitive enough to detect one copy of target sequence in human genomic DNA.

        Contact: Wen Zhou, wzhou@

        cellsig_cfxrt.jpg

        Nanodrop 2000

        Micro-volume full spectrum UV-Visible spectrophotometer for sample volumes as low as 0.5 µL.

        Contact: Dan Zhou, dzhou@

        Nanodrop 2000

        QIAcube

        Fully automated sample preparation of Qiagen spin-column kits (up to 12 samples per run) for purification of DNA, RNA or proteins.

        Contact: Xun Wang, xwang2@

        QIAcube

        I2500 KC Incubator Shaker

        Floor-mounted shaker accepts up to 6-L flasks for culture.

        Contact: Terry Kim, tdkim@

        I2500 KC Incubator Shaker

        Innova 43 Incubator Shaker

        Floor-mounted temperature-controlled shaker accepts up to 6-L flasks for culture. Can go down to 5°C.

        Contact: Terry Kim, tdkim@

        Innova 43 Incubator Shaker

        Avanti J-26 Centrifuge

        Centrifuge up to 6 1-Liter cultures.

        Contact: Alex Siegel, asiegel@

        Avanti J-26 Centrifuge

        Ultrospec 10

        Portable spectrophotometer for OD600 readings.

        Contact: John Thompson, jthompso@

        Ultrospec 10

        -80°C freezer

        Intended for use as a back-up of individual lab freezers.

        Contact: Brendan O'Boyle, bmoboyle@

        -80°C freezer

        Typhoon FLA 9000

        High-resolution biomolecular imager capable of detecting storage phosphor, chemiluminescence, and multiplex fluorescence with 473 nm, 532 nm, and 625 nm excitation. Analysis done with ImageQuant software.

        Contact: Siobhan MacArdle, smacardl@

        Typhoon FLA 9000

        Amaxa 4D-Nucleofector

        Efficient electroporation of mammalian cells for transfection.

        Contact: John Thompson (Hsieh-Wilson), x8119, jthompso@

        Amaxa 4D-Nucleofector

        Neon Transfection System

        High efficiency electroporation transfection device for primary cells, stem cells, and difficult-to-transfect cells. It is capable of transfecting 10 or 100 µL volumes.

        Contact: Alison E Ondrus/Ondrus Lab, aondrus@

        Neon Transfection System

        Nikon SMZ1000 Microscope

        Used for dissections and imaging (transmitted light) with the camera attached. Zoom from 0.8 to 8X, in a clean bench to provide a sterile dissection/preparation work space.

        Contact: John Thompson, jthompso@

        Nikon SMZ1000 Microscope

        Nikon TS100 Microscope

        Used for basic visualization of cultured cells at 10 and 20X, allowing for monitoring and counting cells.

        Contact: Kun Mmiao, kmiao@

        Nikon TS100 Microscope

        Clean Bench

        Provides sterile environment for sensitive dissections or microscope work.

        Contact: John Thompson (Hsieh-Wilson), x8119, jthompso@

        Clean Bench

        Laminar Flow Hoods/Biosafety Cabinets

        Provides sterile environment for mammalian cell work.

        Contact: Alice Hsu, ahhsu@

        Laminar Flow Hoods

        Mammalian Tissue Culture CO2 Incubator

        Provides a controlled CO2 and temperature environment for cells during dissection, transfection or passaging done at the Center. It is intended for temporary use, and not long term housing of cultured cells.

        Contact: Kun Miao, kmiao@

        Eppendorf Centrifuge 5702

        Tabletop centrifuge that can accomodate 15 or 50 mL conical tubes.

        Contact: Dina Malounda, malounda@

        Eppendorf Centrifuge 5702

        Beckman LS 6000SC Scintillation Counter

        The scintillation counter is designed to provide highly accurate, automated counting of the level of radioactivity in radioactivity-tagged samples.  Instrument used to count radioactive samples.  Note:  New users must complete Radioactivity Safety Training with Haick Issaian (haick.issian@), the Institute Radiation Safety Officer, before getting access to the scintillation counte.

        Contact: Rebekah Silva (Barton), x3202, rsilva@

        thumbnail_Scintillation Counter.jpg

        Mailing address

        Caltech Center for Molecular Medicine
        Division of Chemistry and Chemical Engineering
        MC 164-30
        California Institute of Technology
        Pasadena, CA 91125
        USA